“Infectious symbiology” has been established as a new science direction, which deals with the interactions of microsymbionts and the host in the course of infection under the conditions of their biocommunicative interrelations. Not only is it a technological platform for studying the infectological mechanisms, but also an opportunity to develop new medicines of the symbiotic range. As a part of this new direction, the infectious process is considered as a model for associative symbiosis with its three-vector structure: dominant – host – associant, and the persistent capacities of bacteria is regarded as a “biotarget” for the studying of their regulation and as a “tool” of their biocommunocative interactions.
The concept of associative symbiosis has been developed as a complex aggregated system, which includes: macrosymbiont (host), dominant microsymbiont (the normal flora) and associative microsymbionts (pathogens, opportunistic microorganisms). For the moment it has become possible to determine the significant criteria with the use of which we can evaluate intermicrobial interactions as well as the interactions between prokaryotes and the host. The universal specification of the symbiont interactions has been proven on the basis of various biological models. The research results have been colligated in the “Associative symbiosis” (2007), “Symbiosis and its role during infections” (2011), “Microsymbiocenosis” (2014) monographs.
The idea of colonial resistance of the organism has been elaborated, which is based on the symbiotic interactions of the host organism and the autochthonous microflora, aimed to sustain microecological homeostasis.
The mechanisms of the survival of the microorganisms in course of the carrier state and during the inflammatory infections have been deciphered. It has become possible to discover new biological properties of the microorganisms, which contribute to their persistence in the organism of the host – anticarnozin, antiimmunoglobulin, antilactoferrin, antihemoglobin, antihistone and anticytokin bacterial activities. The materials are colligated in “Ecology of human microorganisms” (2006) and “Mechanisms of survival of bacteria” (2005).
With the use of basic physiological functions of microsymbionts (reproduction and adaptation) the algorithm of “self/non-self” bacterial detection have been developed for the “dominant-associant” pair. Interbacterial foreigness recognition in microsymbiocenosis is conducted on the earlier stage of microorganism interaction, preceding the immune self/non-self differentiation carried by the system of adaptive immunity of the organism.
Primary discrimination of the “foreign” material by the bifidobacteria is the initial stage of the proceeding “signaling” in the regulation of the immune homeostasis in the host.
The universal concept of the pathogenesis of the “system” endogenous bacterial infections has been substantiated, the risk factors for their development have been classified, new methods for the diagnostics and prognosis, treatment and prophylaxis of this pathology have been introduced.
The Award of the Government of the Russian Federation for science and technology has been received for “The development of the technology, establishing the industrial production and introduction into clinical use of the finished dosage form of the new domestic drug “Cycloferon” (the corresponding member of the RAS Bukharin O.V., 2004).
On the basis of the intermicrobial interrelation analysis “lysozyme – antilysozyme”, “histone – antihistone”, “catalase – H2O2” it has become possible to discover the significance of the microsymbiont persistence potential in course of its contact with the host, which participates in the development of the natural biocenosis in the fresh and saline water bodies. New original microbiological methods of ecological evaluation of the water objects have been elaborated and introduced, which allowed to develop the methodical base of bioindication and biomonitoring (“Bioindication of the ecological condition of the lowland rivers”, 2007). The Award of the Government of the Russian Federation for science and technology has been received for the elaboration of the scientific base and introduction of the methods for biomonitoring of the sustainable development of the Volga basin territories” (the corresponding member of the RAS Bukharin O.V., professor Nemtseva N.V., 2010).
As a part of the investigation of the biological diversity of various ecological systems the cataloguing of the human microbiota content has been carried out (“Anaerobic human microflora” (2004), “Biology and ecology of enterococci” (2012)), as well as the cataloguing of natural water bodies algae (“Algal flora of the Orenburg Region”, 2005). Protists species, new for the science and the Orenburg Region, have been isolated and researched.